Detection of transglutaminase activity using click chemistry

detection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts

in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...

Surface Functionalization of Exosomes Using Click Chemistry

A method for conjugation of ligands to the surface of exosomes was developed using click chemistry. Copper-catalyzed azide alkyne cycloaddition (click chemistry) is ideal for biocojugation of small molecules and macromolecules to the surface of exosomes, due to fast reaction times, high specificity, and compatibility in aqueous buffers. Exosomes cross-linked with alkyne groups using carbodiimid...

Functionalization of polymer microspheres using click chemistry.

We describe a new method to covalently link a wide variety of molecules to the surface of colloidal polymer microspheres using the Cu(I)-catalyzed azide-alkyne reaction, most commonly known under the class of reactions identified by the term click chemistry. The method is generic and readily applied to a spectrum of colloidal particle systems allowing surfaces to be tailored with virtually any ...

Click chemistry with DNAw

Click Chemistry background information

Especially biomolecule labeling requires reaction procedures that can be performed under physiological conditions (neutral pH, aqueous solution, ambient temperature) with low reactant concentrations to ensure non-toxic, low background labeling at reasonable time scales while still preserving biological function. Among the plethora of possible reactions only a few generally fit the necessary rea...